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RESOURCES

Webinar Recordings

www.hdxms.net/WebinarRecordings  

 

USEFUL EXTERNAL LINKS AND ONLINE RESOURCES FOR HDX-MS

http://www.hxms.com/hxms.htm

http://peterslab.org/MSTools/

http://hx2.med.upenn.edu/download.html

HDX viewer: http://proteomique.ipbs.fr:8080/

HDXmodeller – A fully automatic web-server for high resolution HDX-MS: https://hdxsite.nms.kcl.ac.uk/

Community-maintained list of HDX-MS software, experimental protocols, and analytics guidelines: https://github.com/hadexversum/HDX-MS-resources

HX-Express v3

Download here: HXExpressv3.zip

  Example Data:  Raw Data: DRVYIHPF_z2.xlsx Processed Data: DRVYIHPF_z2_processed.xlsx Time Legend: Timepoint_Legend.xlsx   Video tutorial: https://www.youtube.com/watch?v=NyPSr7egw6w   If you use HX-Express v3 then please cite the following publication: Tuttle LM, James EI, Georgescauld F, Wales TE, Weis DD, Engen JR, Nath A, Klevit RE, Guttman M (2025) Rigorous Analysis of Multimodal HDX-MS Spectra, J. Am. Soc. Mass Spectrom. 36(2):416-423. doi: 10.1021/jasms.4c00471  

USEFUL HDX-MS protocols (in pdf/excel) contributed from society members:

Lab of Kasper Rand: https://pharmacy.ku.dk/research/protein-analysis-group/

ES_12_Pepsin column repacking_v 2.0Download

DA_03_Statistical Analysis of differential HDX_v1.7Download

DA_07_HXPep_v 1.0Download

SP_03_HDX-MS Sample Prep-v1.2Download

SP_11_How to Pack a Micro Trap Column_v 1.5Download

SP_13_In-solution pepsin digestion using agarose beads_v 1.0Download

SP_17_Back Exchange UPLC HDX System Suitability Test_v 1 1Download